11 Beta- Hydroxysteroid Dehydrogenase Activity In Feline, Equine, And Ossabaw Swine Adipose Tissue

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Author: Fabiana H.G. Farias

Adviser: Duane H. Keisler

Animal Sciences, MS
SS 2007

 

Public Abstract: A study was conducted to determine if 11Beta HSD1 activity existed in adipose tissue in a species dependent manner and if so, did the amount of enzymatic activity differ with adipose depot within: feline, equine, or porcine. Feline: fat samples were collected from 6 cats, each sampled from 5 different adipose depots: subcutaneous midline (SQM), subcutaneous inguinal (SQI), omental (OM), falciform (FAL), and retro- peritoneal (RP). Immunohistochemical analysis revealed the presence of 11&#946;HSD1 in each of the adipose depots examined. Level of 11Beta HSD1 activity differed with adipose depot and cat tested (P less than 0.05): SQM greater than SQI, RP, or OM. Morphometric analysis of each adipose depot also revealed that adipocyte diameters differed (P < 0.05) with adipose depot and cat. SQI Adipocyte diameters for SQI > FAL or OM, while RP > FAL, OM, or SQM. Regardless, adipocyte volume did not correlate with 11 Beta HSD1 enzymatic activity. Equine: fat samples were collected from abdominal and subcutaneous adipose depots from each of 23 horses. Immunohistochemical analysis confirmed the presence of 11 Beta-HSD1 in equine, but activity level did not differ between subcutaneous and adipose tissue depots, nor did enzyme activity differ with body condition score of the horse. Porcine: fat samples were collected from abdominal and subcutaneous adipose depots from 8 Ossabaw pigs. Immunohistochemical analysis confirmed the presence of 11 Beta-HSD1 in porcine, but the level of activity did not differ between subcutaneous vs. adipose tissue depots.